COMPLEX CONNECTIONS OF KUPRUM (II) WITH AMINOSPIRTS AS ACTIVATORS OF HEPATOCYTES PROLIFERATION

A. L. Kovalenko, T. A. Kizimyshina

Abstract


Complex compounds Cuprum (II) with amino alcohols have been studied as growth promoters for animal and human cells. In practical medicine, there is the problem of stimulating growth cells of animals and humans both from the point of view of regeneration of organs and tissues, and from the standpoint of obtaining cellular material and its use for organ transplantation, as well as for obtaining from cells grown outside the body, various biologically active substances. The main functional cells of the liver (hepatocytes) of healthy, adult individuals have an insignificant degree of proliferative activity. The most important problem of modern chemical pathology is the management of the processes of proliferation of liver cells. There are two types of substances that activate the processes of proliferation – natural and synthetic activators. The search for substances activating the processes of proliferation is carried out in two directions – obtaining natural activators from animal tissues and organs, plant tissues, and obtaining synthetic drugs that have the ability to enhance the proliferative properties of cells. Since the process of obtaining natural activators is complex and expensive, it made sense to synthesize and investigate low-cost and publicly available substances of chemically and biologically active compounds, which we studied earlier. A number of complex compounds of biometal with various amino alcohols, such as [Cu(BDDEA)2] X2, [Cu (RDEA-H) Cl]2×nH2O, where BDEAA is benzyldiethanolamine, RDEA-N are derivatives of diethanolamine, R-C3H5N(ADEA), C3H7N(PDEAZ), C4H7N(MADEA); X - SO42−; NO3. For studies, the dependence of the action of the concentration of complexes on time was used. In the role of the test system was used monolayer culture of hepatocytes normal liver of an adult rat. Sampling was carried out at regular intervals. It was found that the complex compounds [Cu (БЗДЭА)2]Сl2 and [Cu (АДЭА-Н)Cl] 2×H2O are pronounced activators of the proliferative properties of the hepatocytes of the liver, and the substance [Cu (MADEA-Н) Cl]2× H2O low concentrations have an inhibitory effect on proliferation, with increasing concentration of DNA – synthesizing hepatocytes. Compared to natural proliferation activators (insulin, vitamin B12), the obtained complex compounds are easy to produce, have low material costs during synthesis and can be recommended for use in practical healthcare and biotechnology.

Keywords


proliferation; liver; complex compounds; hepatocytes

References


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DOI: https://doi.org/10.31319/2519-2884.35.2019.51

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Copyright (c) 2019 A. L. Kovalenko, T. A. Kizimyshina

ISSN (print) 2519-2884

ISSN (online) 2617-8389